Lesley Devine PhD
Associate Research Scientist in Laboratory Medicine; Scientific Director, Cancer Center Immune Monitoring Laboratory
Effects of novel immune therapies on the immune system
The main goal of the lab is in monitoring the cellular immune response to determine the effects on the immune system of treatments with novel immune therapies.Current trials that the core is involved with include anti-CD3 treatment for diabetes and angiogenesis inhibitors and immunomodulatory reagents such as anti-CTLA-4, anti-PD-1 and IL-2 in the treatment of melanoma. 6-color flow cytometry is used for phenotypic analysis of the different immune cell populations, including the use of MHC class I tetramers to measure the antigen-specific response.Functional assays include the use of Luminex, a bead-based technology for multiplex cytokine analysis and flow-based cytotoxicity assays.
- Differential expression of the human CD8beta splice variants and regulation of the M-2 isoform by ubiquitination.
Thakral D, Dobbins J, Devine L, Kavathas PB.
J Immunol. 2008 Jun 1;180(11):7431-42.
- Mapping the binding site on CD8 beta for MHC class I reveals mutants with enhanced binding.
Devine L, Thakral D, Nag S, Dobbins J, Hodsdon ME, Kavathas PB.
J Immunol. 2006 Sep 15;177(6):3930-8.
- Location of the epitope for an anti-CD8alpha antibody 53.6.7 which enhances CD8alpha-MHC class I interaction indicates antibody stabilization of a higher affinity CD8 conformation.
Devine L, Hodsdon ME, Daniels MA, Jameson SC, Kavathas PB.
Immunol Lett. 2004 May 15;93(2-3):123-30.
- The complementarity-determining region-like loops of CD8 alpha interact differently with beta 2-microglobulin of the class I molecules H-2Kb and thymic leukemia antigen, while similarly with their alpha 3 domains.
Devine L, Rogozinski L, Naidenko OV, Cheroutre H, Kavathas PB.
J Immunol. 2002 Apr 15;168(8):3881-6.
- Human CD8 beta, but not mouse CD8 beta, can be expressed in the absence of CD8 alpha as a beta beta homodimer.
Devine L, Kieffer LJ, Aitken V, Kavathas PB.
J Immunol. 2000 Jan 15;164(2):833-8.